This study assesses the environmental impact of reusing glass bottles for mineral water in Italy, contributing to sustainable resource management. Using Life Cycle Assessment (LCA), the study examines the effects of increasing reuse cycles, or “rotations,” on environmental outcomes. Part of a larger project on packaging reuse in Italy, it compares the environmental performance of reusable versus single-use glass bottles, incorporating bottle production, washing, end-of-life, and logistics in the analysis. Data was gathered through detailed inventory questionnaires from Italian mineral water companies using reusable glass bottles, complemented by field visits to two reconditioning plants, to identify the specific impact of reconditioning in the bottle lifecycle.